Article
In vitro and in vivo characterization of putative porcine embryonic stem cells.
Cellular reprogramming - 1 Apr 2010
Vassiliev Ivan, Vassilieva Svetlana, Beebe Luke F S, Harrison Sharon J, McIlfatrick Stephen M, Nottle Mark B
Abstract excerpt
We have developed a new method for the isolation of porcine embryonic stem cells (ESCs) from in vivo-derived and in vitro-produced embryos. Here we describe the isolation and characterization of several ESC lines established using this method. Cells from these lines were passaged up to 14 times, during which they were repeatedly cryopreserved. During this time, ESCs maintained their morphology and continued to...
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