Polymerase chain reaction amplification and sequence analysis of human mutant adenine phosphoribosyltransferase genes: the nature and frequency of errors caused by Taq DNA polymerase.

Abstract excerpt

Using the polymerase chain reaction (PCR) with Taq DNA polymerase, we have amplified a 2.4-kb fragment of genomic DNA containing the adenine phosphoribosyltransferase (APRT) gene from patients with APRT deficiency. Several clones from each patient were sequenced after subcloning the PCR product into M13mp18. Selected regions of the amplified fragment were also sequenced directly. This enabled us to distinguish...

Topics

• Adenine Phosphoribosyltransferase
• Base Sequence
• DNA
• DNA, Single-Stranded
• DNA-Directed DNA Polymerase
• Electrophoresis, Agar Gel
• Humans
• Molecular Sequence Data
• Mutation
• Polymerase Chain Reaction