Article
Inactivation of Campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaA but not flaB is required for invasion.
The EMBO journal - 1 Aug 1991
Wassenaar T M, Bleumink-Pluym N M, van der Zeijst B A
Abstract excerpt
The role of the Campylobacter jejuni flagella in adhesion to, and penetration into, eukaryotic cells was investigated. We used homologous recombination to inactivate the two flagellin genes flaA and flaB of C. jejuni, respectively. Mutants in which flaB but not flaA is inactivated remain motile. In contrast a defective flaA gene leads to immotile bacteria. Invasion studies showed that mutants without motile...
Topics
- Blotting, Southern
- Blotting, Western
- Campylobacter jejuni
- Cell Line
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Flagellin
- Genes, Bacterial
- Microscopy, Electron
- Mutation
- Plasmids