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Article

Isolation of motile and non-motile insertional mutants of Campylobacter jejuni: the role of motility in adherence and invasion of eukaryotic cells.

Molecular microbiology - 1 Dec 1994

Yao R, Burr D H, Doig P, Trust T J, Niu H, Guerry P

Abstract excerpt

A method of insertional mutagenesis for naturally transformable organisms has been adapted from Haemophilus influenzae and applied to the study of the pathogenesis of Campylobacter jejuni. A series of kanamycin-resistant insertional mutants of C. jejuni 81-176 has been generated and screened for loss of ability to invade INT407 cells. Eight noninvasive mutants were identified which showed 18-200-fold reductions...

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PMID: 7715450
DOI: 10.1111/j.1365-2958.1994.tb01324.x

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