Article
Rapid detection of BF haplotypes by a semi-nested polymerase chain reaction, which causes resistance/susceptibility to Marek's disease in chicken.
Scandinavian journal of immunology - 1 Aug 2010
Jin Y-C, Wei P, Wei X-X, Zhao Z-Y, Li Y
Abstract excerpt
A semi-nested polymerase chain reaction (snPCR) assay was developed for the rapid detection of resistant/susceptible BF haplotypes to Marek's disease (MD) using the cDNA samples from peripheral blood leucocytes, liver, spleen and heart from Xiayan homozygous chickens: A(11), C(23), D(8) and D(12) (resistant to MD), A(5) and B(21) (susceptible to MD). The snPCR was utilized to span alternative splicing-out of the...
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