Article
Plant-expressed recombinant mountain cedar allergen Jun a 1 is allergenic and has limited pectate lyase activity.
International archives of allergy and immunology - 1 Jan 2010
Liu Zun, Bhattacharyya Shikha, Ning Bo, Midoro-Horiuti Terumi, Czerwinski Edmund W, Goldblum Randall M, Mort Andrew, Kearney Christopher M
Abstract excerpt
BACKGROUND: Mountain cedar (Juniperus ashei) pollen commonly causes a winter time allergic rhinitis in the central USA. Jun a 1 is the dominant allergenic protein, but biologically active recombinant Jun a 1 has not been successfully expressed, despite numerous attempts with several expression systems. METHOD: Jun a 1 cDNA was inserted into a tobacco mosaic virus vector and transferred to Agrobacterium...
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