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Article

Enzyme immunoassay screening of alpha 1-antitrypsin in dried blood spots from 39 289 newborns.

Clinical chemistry - 1 Jun 1991

Schoos R, Dodinval-Versie J, Verloes A, Lambotte C, Koulischer L

Abstract excerpt

We present a new, simple, and inexpensive sandwich-type double-antibody enzyme immunoassay for alpha 1-antitrypsin in dried blood collected on the fifth day post-partum. The method is very sensitive, having a detection limit of 2.84 fmol/well. Intra- and interassay CVs are 6.1% and 10.3%, respectively, for assay of 5-mm-diameter blood spots eluted into 7 mL of phosphate buffer. Since February 1984, we have used...

Topics

Enzyme-Linked Immunosorbent Assay
Humans
Infant, Newborn
Isoelectric Focusing
Liver Diseases
Phenotype
alpha 1-Antitrypsin
alpha 1-Antitrypsin Deficiency

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 2049845

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2049845-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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