Article
Transcript profiling of two alfalfa genotypes with contrasting cell wall composition in stems using a cross-species platform: optimizing analysis by masking biased probes.
BMC genomics - 24 May 2010
Yang S Samuel, Xu Wayne Wenzhong, Tesfaye Mesfin, Lamb JoAnn F S, Jung Hans-Joachim G, VandenBosch Kathryn A, Vance Carroll P, Gronwald John W
Abstract excerpt
BACKGROUND: The GeneChip(R) Medicago Genome Array, developed for Medicago truncatula, is a suitable platform for transcript profiling in tetraploid alfalfa [Medicago sativa (L.) subsp. sativa]. However, previous research involving cross-species hybridization (CSH) has shown that sequence variation between two species can bias transcript profiling by decreasing sensitivity (number of expressed genes detected) and...
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