Article
Identity determinants of E. coli tRNA(Val).
Biochemical and biophysical research communications - 14 Jun 1991
Tamura K, Himeno H, Asahara H, Hasegawa T, Shimizu M
Abstract excerpt
In order to study the identity elements of valine tRNA, various transcripts of E. coli valine tRNA mutants were constructed. Both mutations at the second letter A35 of the anticodon and at the discriminator base A73 seriously damaged valine charging activity. Mutations at either the G3-C70 or U4-A69 base pairs in the acceptor stem also affected the activity. Only one nucleotide substitution of the second letter...
Topics
- Base Composition
- Base Sequence
- Binding Sites
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA, Transfer, Ala
- RNA, Transfer, Val
- Valine-tRNA Ligase