Article
Development of a specific TaqMan real-time PCR assay for quantification of Fusarium graminearum clade 7 and comparison of fungal biomass determined by PCR with deoxynivalenol content in wheat and barley.
International journal of food microbiology - 30 Jun 2010
Demeke Tigst, Gräfenhan Tom, Clear Randy M, Phan Anh, Ratnayaka Indira, Chapados Julie, Patrick Susan K, Gaba Don, Lévesque C André, Seifert Keith A
Abstract excerpt
A Fusarium graminearum clade 7 specific real-time quantitative PCR (qPCR) assay was developed in this study based on unique polymorphisms in sequences of the mating type protein (MAT) gene. PCR amplification was not observed in eight phylogenetic lineages of the F. graminearum complex and four other closely related Fusarium species. Accuracy of the quantification of the real-time PCR assay was verified with wheat...
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