Article
Genetic screening identifies cyanogenesis-deficient mutants of Lotus japonicus and reveals enzymatic specificity in hydroxynitrile glucoside metabolism.
The Plant cell - 1 May 2010
Takos Adam, Lai Daniela, Mikkelsen Lisbeth, Abou Hachem Maher, Shelton Dale, Motawia Mohammed Saddik, Olsen Carl Erik, Wang Trevor L, Martin Cathie, Rook Fred
Abstract excerpt
Cyanogenesis, the release of hydrogen cyanide from damaged plant tissues, involves the enzymatic degradation of amino acid-derived cyanogenic glucosides (alpha-hydroxynitrile glucosides) by specific beta-glucosidases. Release of cyanide functions as a defense mechanism against generalist herbivores. We developed a high-throughput screening method and used it to identify cyanogenesis deficient (cyd) mutants in the...
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