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Development and Evaluation of a Sensitive PCR-ELISA System for Detection of Schistosoma Infection in Feces

20 Apr 2010

Abstract excerpt

BACKGROUND: A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed to overcome the need for sensitive techniques for the efficient diagnosis of Schistosoma infection in endemic settings with low parasitic burden. METHODOLOGY/PRINCIPAL FINDINGS: This system amplifies a 121-base pair tandem repeat DNA sequence, immobilizes the resultant 5' biotinylated product on streptavidin-coated strip-well...

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PMID: 20421918
DOI: 10.1371/journal.pntd.0000664

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