Article
Rapid DNA extraction of pig ear tissues.
Meat science - 1 Jul 2010
Kunhareang S, Zhou H, Hickford J G H
Abstract excerpt
A single-step DNA isolation procedure from pig tissues was developed and the product used directly for polymerase chain reaction (PCR) amplification, single-strand conformational polymorphism (SSCP) analysis and sequencing. The procedure consists of proteinase K digestion of 2-10mg of fresh tissue, at 55 degrees C for 1h, followed by application of the products of digestion to filter paper. A 1.2mm-diameter punch...
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