Article
Involvement of recF in 254 nm ultraviolet radiation resistance in Deinococcus radiodurans and Escherichia coli.
Current microbiology - 1 Nov 2010
Chang Xiaosong, Yang Lan, Zhao Qing, Fu Wenjuan, Chen Hao, Qiu Zhiqun, Chen Ji-an, Hu Ran, Shu Weiqun
Abstract excerpt
recF is a critical gene involved in the RecFOR pathway of DNA repair in Deinococcus radiodurans (D. radiodurans). To investigate the role of recF in UV-C radiation resistance, we generated the recF-deficient D. radiodurans strain R1, expressed recF in Escherichia coli (E. coli) BL21 cells, and compared the ability of each to resist UV-C radiation by F (10), inactivation constant (IC), and extrapolation number...
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