Article
Quantitative analysis of cell surface membrane proteins using membrane-impermeable chemical probe coupled with 18O labeling.
Journal of proteome research - 7 May 2010
Zhang Haizhen, Brown Roslyn N, Qian Wei-Jun, Monroe Matthew E, Purvine Samuel O, Moore Ronald J, Gritsenko Marina A, Shi Liang, Romine Margaret F, Fredrickson James K, Pasa-Tolić Ljiljana, Smith Richard D, Lipton Mary S
Abstract excerpt
We report a mass spectrometry-based strategy for quantitative analysis of cell surface membrane proteome changes. The strategy includes enrichment of surface membrane proteins using a membrane-impermeable chemical probe followed by stable isotope (18)O labeling and LC-MS analysis. We applied this...
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