Article
Transcriptional and proteomic analysis of a ferric uptake regulator (fur) mutant of Shewanella oneidensis: possible involvement of fur in energy metabolism, transcriptional regulation, and oxidative stress.
Applied and environmental microbiology - 1 Feb 2002
Thompson Dorothea K, Beliaev Alexander S, Giometti Carol S, Tollaksen Sandra L, Khare Tripti, Lies Douglas P, Nealson Kenneth H, Lim Hanjo, Yates John, Brandt Craig C, Tiedje James M, Zhou Jizhong
Abstract excerpt
The iron-directed, coordinate regulation of genes depends on the fur (ferric uptake regulator) gene product, which acts as an iron-responsive, transcriptional repressor protein. To investigate the biological function of a fur homolog in the dissimilatory metal-reducing bacterium Shewanella oneidensis MR-1, a fur knockout strain (FUR1) was generated by suicide plasmid integration into this gene and characterized...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Electrophoresis, Gel, Two-Dimensional
- Energy Metabolism
- Gene Expression Profiling
- Gene Expression Regulation, Bacterial
- Iron