Article
[Establishment of a fluorescent real-time quantitative RT-PCR assay for detection of genotype 4 hepatitis E virus in swine stools].
Bing du xue bao = Chinese journal of virology - 1 Jan 2010
Jia Peng, Jin Ning-Yi, Li Xiao, Zhu Guang-Ze, Liu Yan, Gao Peng, Xu Xiao-Hong, Yang En-Cheng, Meng Ri-Zeng, Kan Shi-Fu
Abstract excerpt
The primers and probes for the Real-time RT-PCR were designed based on the multiple sequence (swine and humans HEV strains) alignments of the ORF3 region of genotype 4 HEV. A rapid, sensitive and stable TaqMan Real-time RT-PCR assay was established, and its specificity and sensitivity were assessed, and comparison of the Real-time RT-PCR with conventional and nested RT-PCR was performed. The results found that...
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