Article
[Quasispecies investigation on swine hepatitis E viruses].
Bing du xue bao = Chinese journal of virology - 1 Jan 2010
Zhang Xi-Ying, Zhu Rui-Liang, Zhu Yu-Min, Dong Shi-Juan, Yu Rui-Song, Shen Shi-Yuan, Wang Zhen-Yong, Li Zhen
Abstract excerpt
The objective of current study was to investigate the quasispecies of hepatitis E virus in swine. The partial ORF2 region of HEV envelope gene from four swine HEV strains was amplified by RT-nested polymerase chain reaction (RT-nPCR). After cloning and transformation of PCR products, 20 positive clones of each HEV isolate were subject to sequencing and DNA analysis. The homology among the different clones of each...
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