Article
Extended C-terminal tail of wheat histone H2A interacts with DNA of the "linker" region.
Journal of molecular biology - 20 Apr 1991
Lindsey G G, Orgeig S, Thompson P, Davies N, Maeder D L
Abstract excerpt
The preparation of hybrid histone octamers with wheat histone H2A variants replacing chicken H2A in the chicken octamer is described. The fidelity of the reconstituted hybrid octamers was confirmed by dimethyl suberimidate cross-linking. Polyglutamic-acid-mediated assembly of these octamers on long DNA and subsequent micrococcal nuclease (MNase) digestion demonstrated that, whereas chicken octamers protected 167...
Topics
- Amino Acid Sequence
- Animals
- Base Composition
- Chickens
- DNA
- Genetic Variation
- Histones
- Molecular Sequence Data
- Protein Conformation
- Protein Denaturation
- Triticum