Article
Novel microRNAs in silkworm (Bombyx mori).
Functional & integrative genomics - 1 Aug 2010
Cai Yimei, Yu Xiaomin, Zhou Qing, Yu Caixia, Hu Haiyan, Liu Jiucheng, Lin Hongbin, Yang Jin, Zhang Bing, Cui Peng, Hu Songnian, Yu Jun
Abstract excerpt
We acquired more than 4 million useful sequences using a high-throughput method from a library for miRNA identification, which is constructed from a mixture of 14 RNA samples from different developmental stages. We mapped 247,410 reads to known silkworm miRNAs in miRBase (13.0), 701,913 reads to other RNA molecules based on sequence homology, and 3,219,395 reads to the silkworm genome. Our analysis identified 54...
Topics
- Animals
- Base Sequence
- Bombyx
- Cluster Analysis
- Conserved Sequence
- Gene Expression Regulation
- Gene Library
- MicroRNAs
- Molecular Sequence Data
- Nucleic Acid Conformation
- Polymorphism, Single Nucleotide
- Reproducibility of Results
- Reverse Transcriptase Polymerase Chain Reaction