Article
Rational engineering of Thermus thermophilus cytochrome c552 to a thermally tolerant artificial peroxidase.
Dalton transactions (Cambridge, England : 2003) - 28 Mar 2010
Nakajima Hiroshi, Ramanathan Kalaivani, Kawaba Naomi, Watanabe Yoshihito
Abstract excerpt
In a previous study, we constructed a prototype thermally tolerant artificial peroxidase from an electron transfer protein, cytochrome c(552) from Thermus thermophilus, and demonstrated that engineering of proteins from thermophiles could be a promising methodology to produce artificial enzymes for practical use. In the present study, further improvement of the prototype (the V49D/M69A mutant) in enzymatic...
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