Undifferentiated Propagation of the Human Embryonic Stem Cell Lines, H1 and HSF6, on Human Placenta-Derived Feeder Cells Without Basic Fibroblast Growth Factor Supplementation

Abstract excerpt

In order for human embryonic stem cells (hESCs) to be cultured on mouse embryonic fibroblast (MEFs) feeder cells, continuous basic fibroblast growth factor (bFGF) supplementation is required. However, the role of bFGF in a culture system using human-derived feeder cells has not been evaluated until now. In this study, we propagated the widely used hESC lines, H1 and HSF6, on human placenta-derived feeder cells...

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