Identification of Orthopaedic Infections Using Broad-Range Polymerase Chain Reaction and Reverse Line Blot Hybridization

Abstract excerpt

BACKGROUND: Culture remains the gold standard in the diagnosis of bacterial infection, but molecular biological techniques have yielded promising results. In this study, we validated a combined polymerase chain reaction and reverse line blot hybridization protocol for identifying musculoskeletal infections. METHODS: Samples were obtained from seventy-six patients undergoing orthopaedic surgery for various aseptic...

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