Article
Novel method to detect a construct-specific sequence of the acetolactate synthase gene in genetically-modified flax CDC Triffid (FP967).
Biological & pharmaceutical bulletin - 1 Jan 2010
Nakamura Kosuke, Akiyama Hiroshi, Yamada Chihiro, Satoh Rie, Makiyama Daiki, Sakata Kozue, Kawakami Hiroshi, Mano Junichi, Kitta Kazumi, Teshima Reiko
Abstract excerpt
During the fall of 2009, a trace of unauthorized genetically modified (GM) flax (Linum usitatissimum L.) line, CDC Triffid, which is resistant to sulfonylurea herbicides, was detected in many countries including Japan. A method to reliably identify the CDC Triffid line was urgently required. We developed a novel construct-specific real-time polymerase chain reaction (PCR) method to identify the mutant...
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