Rapid detection of <i>Orthopoxvirus</i> by semi‐nested PCR directly from clinical specimens: A useful alternative for routine laboratories

Abstract excerpt

Orthopoxvirus (OPV) has been associated with worldwide exanthematic outbreaks, which have resulted in serious economic losses as well as impact on public health. Although the current classical and molecular methods are useful for the diagnosis of OPV, they are largely inaccessible to unsophisticated clinical laboratories. The major reason for the inaccessibility is that they require both virus isolation and DNA...

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