Article
Genotyping of Candida albicans using length fragment and high-resolution melting analyses together with minisequencing of a polymorphic microsatellite locus.
Journal of microbiological methods - 1 Mar 2010
Costa Jean-Marc, Garcia-Hermoso Dea, Olivi Martine, Cabaret Odile, Farrugia Cécile, Lecellier Gaël, Dromer Françoise, Bretagne Stéphane
Abstract excerpt
Microsatellite length polymorphism (MLP) typing is a PCR-based method used for genotyping of the diploid yeast Candida albicans. However, MLP is subject to homoplasia which can hamper the accuracy of the results. We combined fragment length analysis, high-resolution DNA melting (HRM) analysis, and SNaPshot minisequencing after a single amplification of the CDC3 locus to study 95 epidemiologically independent C....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
