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Article

Genetic structure of a Spodoptera frugiperda nucleopolyhedrovirus population: high prevalence of deletion genotypes.

Applied and environmental microbiology - 1 Sept 2004

Simón Oihane, Williams Trevor, López-Ferber Miguel, Caballero Primitivo

Abstract excerpt

A Nicaraguan field isolate (SfNIC) of Spodoptera frugiperda nucleopolyhedrovirus was purified by plaque assay on Sf9 cells. Nine distinct genotypes, A to I, were identified by their restriction endonuclease profiles. Variant SfNIC-B was selected as the standard because its restriction profile corresponded to that of the wild-type isolate. Physical maps were generated for each of the variants. The differences...

Topics

Animals
Cloning, Molecular
Gene Deletion
Genes, Viral
Genetic Variation
Genotype
Nucleopolyhedroviruses
Restriction Mapping
Spodoptera

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 15345446
DOI: 10.1128/AEM.70.9.5579-5588.2004
PMCID: PMC520846

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