Article
The effect of Wag31 phosphorylation on the cells and the cell envelope fraction of wild-type and conditional mutants of Mycobacterium smegmatis studied by visible-wavelength Raman spectroscopy.
Biochemical and biophysical research communications - 1 Jan 2010
Hamasha Khozima, Sahana Moodakare Bheema, Jani Charul, Nyayapathy Seeta, Kang Choong-Min, Rehse Steven J
Abstract excerpt
Non-surface-enhanced Raman spectroscopy using a 514.5 nm wavelength laser has been used to measure the molecular difference of conditional mutants of Mycobacterium smegmatis expressing three different alleles: wild-type wag31(Mtb), phosphoablative wag31T73A(Mtb), and phosphomimetic wag31T73E(Mtb). This study demonstrates that the phosphorylation of Wag31, a key cell-division protein, causes significant...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.