Article
Mutation induced modulation of hydrogen bonding to P700 studied using FTIR difference spectroscopy.
Biochemistry - 26 Aug 2003
Wang Ruili, Sivakumar Velautham, Li Yajing, Redding Kevin, Hastings Gary
Abstract excerpt
Site-directed mutagenesis in combination with Fourier transform infrared difference spectroscopy has been used to study how hydrogen bonding modulates the electronic and physical organization of P700, the primary electron donor in photosystem I. Wild-type PS I particles from Chlamydomonas reinhardtii and a mutant in which ThrA739 is changed to alanine [TA(A739) mutant] were studied. ThrA739 is thought to provide...
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