Article
Characterization of the structure of the phosphoprotein of Chandipura virus, a negative stranded RNA virus probing intratryptophan energy transfer using single and double tryptophan mutants.
Biochimie - 1 Feb 2010
Mukhopadhyay Subhradip, Maity Shyam S, Roy Arunava, Chattopadhyay Dhrubajyoti, Ghosh Kalyan S, Dasgupta Swagata, Ghosh Sanjib
Abstract excerpt
The phosphoprotein (P protein) of Chandipura virus (CHPV), a negative stranded RNA virus, is involved in both transcription and replication phases of the viral life cycle. The two Tryptophan (Trp) residues of CHPV, located at 105 and 135 respectively and two single Trp mutants W135F and W105F and a double Trp mutant W135F/W105F have been characterized by steady state and time-resolved fluorescence and...
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