Article
The detection of genetic variation in Leptospira interrogans serogroup ICTEROHAEMORRHAGIAE by ribosomal RNA gene restriction fragment patterns.
FEMS microbiology letters - 1 Nov 1990
Hookey J V
Abstract excerpt
Deoxyribonucleic acid from Leptospira interrogans serogroup ICTEROHAEMORRHAGIAE reference strains together with clinical and animal isolates were cleaved with EcoRI, electrophoresed and transferred to nylon membranes. An Escherichia coli MRE 600, 16S + 23S ribosomal RNA template was used in the synthesis of a single strand biotin-labelled cDNA probe using a reverse transcriptase. Ribosomal RNA cistrons present in...
Topics
- DNA Probes
- Genes
- Genetic Markers
- Genetic Variation
- Leptospira interrogans
- Phylogeny
- Polymorphism, Restriction Fragment Length
- RNA, Ribosomal
- Restriction Mapping
