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[Analysis of genomic polymorphism in leptospira by polymerase chain reaction with random primers].

Molekuliarnaia genetika, mikrobiologiia i virusologiia - 1 Jan 1998

Pershina M Iu, Shaginian I A, Anan'ina Iu V, Prozorovskiĭ S V

Abstract excerpt

A test system for genetic typing of Leptospirae is developed, based on the polymerase chain reaction (PCR) with arbitrary primers. Thirteen strains of 4 Leptospira species were examined: L. interrogans, L. parva, L. illini, and L. inadai. Analysis of polymorphism of amplicon length (PAL) by the PCR with short Sh1 and Sh2 primers revealed genotypical differences at the inter- and intraspecias levels, as well as at...

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PMID: 9511139

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