Article
Complementation of a pleiotropic Nif-Gln regulatory mutant of Rhodospirillum rubrum by a previously unrecognized Azotobacter vinelandii regulatory locus.
Archives of microbiology - 1 Jan 1990
Hu C Z, Yoch D C
Abstract excerpt
A spontaneous pleiotropic Nif- mutation in Rhodospirillum rubrum has been partially characterized biochemically and by complementation analysis with recombinant plasmids carrying Azotobacter vinelandii DNA in the vicinity of ORF12 [Jacobson et al. (1989) J. Bacteriol 171: 1017-1027]. In addition to being unable to grow on N2 as a nitrogen source the phenotypic characterization of this and other metronidazole...
Topics
- Autoradiography
- Azotobacter
- Bacterial Proteins
- Blotting, Western
- Conjugation, Genetic
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Glutamate-Ammonia Ligase
- Mutation
- Nitrogenase
