Article
Restriction fragment length polymorphism analysis of somatic hybrids between Lycopersicon esculentum and irradiated L. peruvianum: evidence for limited donor genome elimination and extensive chromosome rearrangements.
Molecular & general genetics : MGG - 1 Jul 1990
Wijbrandi J, Zabel P, Koornneef M
Abstract excerpt
The genome composition of asymmetric somatic hybrids, obtained by fusion of leaf protoplasts from Lycopersicon esculentum and gamma-irradiated leaf protoplasts from L. peruvianum, was characterised by Southern blot analysis using 29 restriction fragment length polymorphism markers. Eight "low dose hybrids" and seven "high dose hybrids" (irradiation dose 50 Gray and 300 Gray, respectively) were analysed. By...
Topics
- Alleles
- Blotting, Southern
- Densitometry
- Gamma Rays
- Gene Rearrangement
- Genetic Linkage
- Hybrid Cells
- Plants
- Polymorphism, Restriction Fragment Length
- Protoplasts