Article
Characterization and application of monoclonal antibodies to bovine viral diarrhea virus nonstructural protein 5A.
Archives of virology - 1 Jan 2009
Zahoor Muhammad Atif, Yamane Daisuke, Mohamed Yassir Mahgoub, Kobayashi Kyousuke, Kato Kentaro, Tohya Yukinobu, Akashi Hiroomi
Abstract excerpt
Bovine viral diarrhea virus (BVDV) nonstructural protein 5A (NS5A) is one the least studied of the BVDV proteins. Therefore, to develop a tool for unraveling the functions performed by BVDV NS5A, monoclonal antibodies (MAbs) were generated by fusion of myeloma cells with spleen cells from mice immunized with recombinant E. coli-expressed GST-NS5A protein. Two MAbs (1H12 and 2F9) were established on the basis of...
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