Article
Whole genome amplification (WGA) for archiving and genotyping of clinical isolates of Cryptosporidium species.
Parasitology - 1 Jan 2010
Bouzid Maha, Heavens Darren, Elwin Kristin, Chalmers Rachel M, Hadfield Stephen J, Hunter Paul R, Tyler Kevin M
Abstract excerpt
Clinical and environmental isolates of pathogens are often unique and may be unculturable, yielding a very limited amount of DNA for genetic studies. Cryptosporidium in particular are difficult to propagate. Whole genome amplification (WGA) is a valuable technique for amplifying genomic material. In this study, we tested 5 WGA commercial kits using Cryptosporidium clinical isolates. DNA of 5 C. hominis and 5 C....
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