Article
Sequence-based genotyping HPV L1 DNA and RNA transcripts in clinical specimens.
Pathology, research and practice - 1 Jan 2009
Satra Maria, Vamvakopoulou Dimitra N, Sioutopoulou Despina O, Kollia Panagoula, Kiritsaka Aspasia, Sotiriou Sotirios, Antonakopoulos Georgios, Alexandris Elias, Costantoulakis Pantelis, Vamvakopoulos Nicholas C
Abstract excerpt
We developed a direct sequence-based genotyping method to detect single and multiple HPV L1 DNA and RNA types in genital and dermatological specimens. Our method couples PCR amplification of a highly conserved HPV L1 segment using a broad spectrum-generic primer cocktail mix with automated sequen...
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