Article
PCR-restriction fragment length polymorphism analysis as a tool for Mycobacterium species identification in lepromas for lepromin production.
Leprosy review - 1 Jun 2009
Alban Silvana M, Sella Sandra R B R, Miranda Ruy N, Mira Marcelo Távora, Soccol Vanete Thomaz
Abstract excerpt
OBJECTIVES: The aim of the present work was to standardise a PCR-Restriction Fragment Length Polymorphism analysis (PRA) as a tool to detect the mycobacteriologic composition of lepromas from leprosy patients used in the production of lepromin to improve the quality of the Mitsuda test. DESIGN: PCR-Restriction Fragment Length Polymorphism analysis using hsp65 and rpoB genes were applied to 11 reference strains of...
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