Article
Evaluation of non-radioactive temperature gradient SSCP analysis and of temperature gradient gel electrophoresis for the detection of HPV 6-variants in condylomata acuminata and Buschke-Loewenstein tumours.
European journal of epidemiology - 1 Oct 1995
Rübben A, Traidl C, Baron J M, Grussendorf-Conen E I
Abstract excerpt
A modified non-radioactive single strand conformation polymorphism analysis incorporating a temperature gradient (TG-SSCP) and temperature gradient gel electrophoresis (TGGE) were evaluated for the detection of human papillomavirus type 6 (HPV 6)-variants in 41 condylomata acuminata and 5 Buschke-Loewenstein tumours. TG-SSCP and TGGE analysed part of the transforming ORF E6 of HPV 6 spanning nucleotides 10 to...
Topics
- Condylomata Acuminata
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- Evaluation Studies as Topic
- Genetic Variation
- Humans
- Nucleotides
- Open Reading Frames
- Papillomaviridae
- Papillomavirus Infections
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length