Serial analysis of gene expression: probing transcriptomes for molecular targets.

Abstract excerpt

Serial analysis of gene expression (SAGE) is a method to rapidly count expressed RNA transcripts in a population of cells. The basic approach is to isolate a small transcript tag, clone multiple tags into a sequencing vector and efficiently count the tags by automated sequencing. The result is the fractional representation of nearly every transcript (the transcriptome), in a digital format. These tag counts can...

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