Article
Single nucleotide polymorphism-based diagnostic system for crop-associated Sclerotinia species.
Applied and environmental microbiology - 1 Sept 2009
Andrew Marion, Kohn Linda M
Abstract excerpt
A molecular diagnostic system using single nucleotide polymorphisms (SNPs) was developed to identify four Sclerotinia species: S. sclerotiorum (Lib.) de Bary, S. minor Jagger, S. trifoliorum Erikss., and the undescribed species Sclerotinia species 1. DNAs of samples are hybridized with each of five 15-bp oligonucleotide probes containing an SNP site midsequence unique to each species. For additional verification,...
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