Article
Mutants of the pentose-fermenting yeast Pichia stipitis with improved tolerance to inhibitors in hardwood spent sulfite liquor.
Biotechnology and bioengineering - 1 Dec 2009
Bajwa Paramjit K, Shireen Tasnina, D'Aoust Frédéric, Pinel Dominic, Martin Vincent J J, Trevors Jack T, Lee Hung
Abstract excerpt
Mutants of Pichia stipitis NRRL Y-7124 able to tolerate and produce ethanol from hardwood spent sulfite liquor (HW SSL) were obtained by UV mutagenesis. P. stipitis cells were subjected to three successive rounds of UV mutagenesis, each followed by screening first on HW SSL gradient plates and then in diluted liquid HW SSL. Six third generation mutants with greater tolerance to HW SSL as compared to the wild type...
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