Article
[Multiplex PCR assay for dissemination and diversity of extended-spectrum beta-lactamase genes in Shigella isolates].
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] - 1 Mar 2009
Pu Xiao-ying, Pan Jin-cao, Wang Hao-qiu, Huang Zhi-cheng, Gu Ya-ming
Abstract excerpt
OBJECTIVE: To develop a rapid and simple multiplex polymerase chain reaction (PCR) method which discriminates extended-spectrum beta-lactamases (ESBLs) genes in sporadic Shigella isolates from 1998 to 2007 in Hangzhou city, China. METHODS: After ESBLs screening according to the Clinical and Laboratory Standards Institute (CLSI) method, CTX-M, TEM, SHV and OXA-1 encoding genes were detected by using a multiplex...
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