Article
Characterization of vanadium-binding sites of the vanadium-binding protein Vanabin2 by site-directed mutagenesis.
Biochimica et biophysica acta - 1 Oct 2009
Ueki Tatsuya, Kawakami Norifumi, Toshishige Masaaki, Matsuo Koichi, Gekko Kunihiko, Michibata Hitoshi
Abstract excerpt
BACKGROUND: Vanabins are a unique protein family of vanadium-binding proteins with nine disulfide bonds. Possible binding sites for VO2+ in Vanabin2 from a vanadium-rich ascidian Ascidia sydneiensis samea have been detected by nuclear magnetic resonance study, but the metal selectivity and metal-binding ability of each site was not examined. METHODS: In order to reveal functional contribution of each binding...
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