Performance of Real-Time Reverse Transcription Polymerase Chain Reaction for the Detection of <i>Foot-and-Mouth Disease Virus</i> during Field Outbreaks in the United Kingdom in 2007

Abstract excerpt

Rapid and accurate diagnosis is essential for effective control of foot-and-mouth disease (FMD). The present report describes the practical steps undertaken to deploy a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) to process the samples received during the outbreaks of FMD in the United Kingdom in 2007. Two independent real-time RT-PCR assays targeting different regions (5'UTR and...

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