Article
Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector.
Infection and immunity - 1 Dec 1991
Donnenberg M S, Kaper J B
Abstract excerpt
The ability to attach to epithelial cells, efface the microvillus surface, and disrupt the underlying cytoskeleton is characteristic of enteropathogenic Escherichia coli (EPEC). Recently, eae, a gene necessary for this phenomenon, was described (A. E. Jerse, J. Yu, B. D. Tall, and J. B. Kaper, Proc. Natl. Acad. Sci. USA 87:7839-7843, 1990). We report the use of a novel suicide vector containing the pir-dependent...
Topics
- Bacterial Adhesion
- Bacterial Outer Membrane Proteins
- Base Sequence
- Chromosome Deletion
- Escherichia coli
- Genes, Bacterial
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Plasmids