Article
Detection and genotyping analysis of human papillomavirus isolates from liquid-based cervical cytology specimens.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Avissar Patricia, Malinowski Douglas P
Abstract excerpt
The detection of oncogenic subtypes of human papillomavirus (HPV) from exfoliated cervical cells collected in liquid-based preservative fluid at the time of the annual Pap test can be accomplished using PCR-based amplification methods. DNA sequence analysis of the L1 open reading frame (ORF) permits determination of the HPV viral subtype. DNA sequence analysis of the E6 and E7 ORFs permits further detection of...
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