Article
Screening for cystic fibrosis: feasibility of molecular genetic analysis of dried blood specimens.
Biochemical medicine and metabolic biology - 1 Aug 1991
Seltzer W K, Accurso F, Fall M Z, VanRiper A J, Descartes M, Huang Y, McCabe E R
Abstract excerpt
Direct genotypic analysis for the common Caucasian cystic fibrosis mutation (delta F508) was performed using dried blood specimens in a filter paper matrix (neonatal screening blotter). DNA was obtained from dried and liquid blood samples, amplified, and analyzed by polyacrylamide gel electrophoresis. Additionally, intact 4-mm-diameter punched discs from blotters containing dried blood specimen were used in the...
Topics
- Cystic Fibrosis
- DNA
- DNA Mutational Analysis
- Genotype
- Humans
- Infant, Newborn
- Mass Screening