Article
Strategies for undertaking expressed sequence tag (EST) projects.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Clifton Sandra W, Mitreva Makedonka
Abstract excerpt
Complementary DNA (cDNA) sequencing can be used to sample an organism's transcriptome, and the generated EST sequences can be used for a variety of purposes. They are especially important for enhancing the utility of a genome sequence or for providing a gene catalog for a genome that has not or will not be sequenced. In planning and executing a cDNA project, several criteria must be considered. One should clearly...
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