Article
Genotyping Campylobacter jejuni by comparative genome indexing: an evaluation with pulsed-field gel electrophoresis and flaA SVR sequencing.
Foodborne pathogens and disease - 1 Apr 2009
Pittenger Lauren G, Englen Mark D, Parker Craig T, Frye Jonathan G, Quiñones Beatriz, Horn Sharon T, Son Insook, Fedorka-Cray Paula J, Harrison Mark A
Abstract excerpt
Comparative genome indexing (CGI) using whole-genome DNA microarrays was evaluated as a means of genotyping Campylobacter jejuni relative to two standard methods, pulsed-field gel electrophoresis (PFGE) and flaA short variable region sequencing (flaA SVR typing). Thirty-six geographically diverse C. jejuni isolates were selected from a collection of cattle and chicken isolates. The BioNumerics software program...
Topics
- Animals
- Bacterial Typing Techniques
- Campylobacter jejuni
- Cattle
- Chickens
- DNA, Bacterial
- Electrophoresis, Gel, Pulsed-Field
- Flagellin
- Genome, Bacterial
- Genotype
- Oligonucleotide Array Sequence Analysis
- Sequence Analysis, DNA